Feb 10, 2021 (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1: 250, AB13847, Abcam, MA), respectively. Rat anti- mouse CD3
(cat. no. ab13847; 1:100; Abcam)] was added, and the nega-tive control group was incubated with PBS. The sample was placed in a wet box overnight at 4˚C. The sample was then .
Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE. ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Antibody Information. Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3, anti- active Caspase 3. Clonality: polyclonal antibody.
The treated H9c2 cells were harvested, and the treated myocardial tissues of rats were collected and ground on the ice. After washing, the samples were combined with the RIPA lysate (BIOYKSW, cat. no. orb-EYK024573) containing 1% PMSF (Solarbio, cat.
ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application.
Gene CASP3 Modification Unmodified View Product On Supplier's Website Request a Quote from Abcam. Supplier suggested.
Structured Review. Abcam expose mouse and rabbit specific hrp dab detection ihc kit. https://www.bioz.com/result/expose mouse and rabbit specific hrp dab detection ihc kit/product/Abcam. Average 97 stars, based on 3 article reviews. Price from $9.99 to $1999.99. expose mouse and rabbit specific hrp dab detection ihc kit - by Bioz Stars , 2020-12.
The cell cloning, proliferation, apoptosis 2020-09-03 · After blocking for 2 h in TBST containing 5% non-fat milk, the membranes were incubated overnight at 4 °C with the following primary antibodies: Caspase-3 (#ab13847, Abcam, Cambridge, UK), β-actin(# ab179467, Abcam), ERK (#4695, Cell Signaling Technology, Danvers, MA, USA), Phospho-ERK (#4370, Cell Signaling Technology) diluted in 5% non-fat milk containing TBST. Digital data.
I'm not able to detect cleaved caspase-3 by western blotting using the ab13847 antibody. My wb input are lysed primary cortical neurons treated with staurosporine 1 uM for 4 and 6 h.
Vägmärket cykelöverfart
no. ab66705; 1:2,000; Abcam), anti-TNF-α (cat. no.
Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973
Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation.
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active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973
Average 97 stars, based on 3 article reviews. Price from $9.99 to $1999.99. expose mouse and rabbit specific hrp dab detection ihc kit - by Bioz Stars , 2020-12. 2018-04-03 2021-02-08 ab13847 was shown to recognise Caspase 3 when Caspase 3 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.
2020-01-30
After washing, the samples were combined with the RIPA lysate (BIOYKSW, cat. no. orb-EYK024573) containing 1% PMSF (Solarbio, cat. no. IP0280). Inflammation drives atherosclerotic plaque progression and rupture, and is a compelling therapeutic target. Consequently, attenuating inflammation by reducing local macrophage accumulation is an appealing approach.
Anti-rabbit Alexa Fluor 488, Life technologies BAK (D4E4, #12105), and Hsp60 (#4870); from Abcam: active caspase 3 (#ab13847), BCL-xL (#ab32370, used at 1/500 dilution), BCL-2 (#ab18210, used at DsRed (MBL, PM005, 1: 1000) och aktiverad-caspase3 (Abcam, ab13847, 1: 1000). Bilder av uncropped western blottings visas i kompletterande figur 12. ab13248), anti-Bach2 (1: 500, ab83364), anti-caspase 3 (1: 1000, ab13847), anti-Nrf2 (1 : 1000, ab31163), anti-Bcl-2 (1: 1000, 12, 789–1-AP), anti-Maf (1: 500 UK), kanin-anti-aktivt caspase-3 (ab13847, 1: 200; Abcam), mus-anti-a-tubulin (klon B5.1.2, 1: 2000; Sigma-Aldrich), get-anti-RanBP1 (C-19) (sc-1160, 1:25; overnight with rabbit anti-active Caspase 3 (Casp3) polyclonal antibodies (1:1000, ab13847, Abcam Co. Ltd.) or rabbit anti-Fas (1:1000, ab82419, Abcam Co. som användes omfattade kanin anti-LC3-antikropp (4108, Cell Signaling, USA), kanin-antiaktiva caspase-3 polyklonala antikroppar (ab13847, Abcam Co. ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.